Figure 1.

ZK1, ZK2 and ZK6 cell lines are MARCO^-/- and SR-AI/II^-/- (MS^-/-) by PCR genotyping. With primers for SR-A, amplifies a 325 bp DNA fragment from the C57BL/6 wild-type (WT) allele; with SR-AI/II mutant allele primers, amplifies a 434 bp DNA fragment from SRA-deficient ZK1, ZK2 and ZK6 cells. With primers for MARCO wild-type allele, amplifies a 500 bp DNA fragment from WT mice; with primers for MARCO mutant allele, amplifies a 850 bp DNA fragment from ZK cells. ZK1, ZK2 and ZK6 clones exhibited both MARCO and SRA-I/II-deficient. PCR products, ca.10 μl/each was resolved on a 1.5% agarose gel by gel electrophoresis. M, 100 bp DNA marker.