Open Access Research

Cellular uptake and localization of inhaled gold nanoparticles in lungs of mice with chronic obstructive pulmonary disease

Marianne Geiser1*, Oliver Quaile1, Alexander Wenk2, Christoph Wigge1, Sylvie Eigeldinger-Berthou1, Stephanie Hirn25, Martin Schäffler2, Carsten Schleh26, Winfried Möller2, Marcus A Mall34 and Wolfgang G Kreyling27

Author Affiliations

1 Institute of Anatomy, Medical Faculty, University of Bern, CH-3012 Bern, Switzerland

2 Institute of Lung Biology and Disease/Comprehensive Pneumology Center, Helmholtz Center Munich – German Research Center for Environmental Health, D-85764 Neuherberg/Munich, Germany

3 Department of Translational Pulmonology, Translational Lung Research Center, Member of the German Center for Lung Research, University of Heidelberg, D-69120 Heidelberg, Germany

4 Division of Pediatric Pulmonology & Allergy and Cystic Fibrosis Center, Department of Pediatrics ІІІ, University of Heidelberg, D-69120 Heidelberg, Germany

5 Current address: Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians-University, D-81377 Munich, Germany

6 Current address: BSL BIOSERVICE Scientific Laboratories GmbH, D-82152 Planegg/Munich, Germany

7 Current address: Institute of Epidemiology 2, Helmholtz Center Munich – German Research Center for Environmental Health, D-85764 Neuherberg, Munich, Germany

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Particle and Fibre Toxicology 2013, 10:19  doi:10.1186/1743-8977-10-19

Published: 16 May 2013

Abstract

Background

Inhalative nanocarriers for local or systemic therapy are promising. Gold nanoparticles (AuNP) have been widely considered as candidate material. Knowledge about their interaction with the lungs is required, foremost their uptake by surface macrophages and epithelial cells.

Diseased lungs are of specific interest, since these are the main recipients of inhalation therapy. We, therefore, used Scnn1b-transgenic (Tg) mice as a model of chronic obstructive pulmonary disease (COPD) and compared uptake and localization of inhaled AuNP in surface macrophages and lung tissue to wild-type (Wt) mice.

Methods

Scnn1b-Tg and Wt mice inhaled a 21-nm AuNP aerosol for 2 h. Immediately (0 h) or 24 h thereafter, bronchoalveolar lavage (BAL) macrophages and whole lungs were prepared for stereological analysis of AuNP by electron microscopy.

Results

AuNP were mainly found as singlets or small agglomerates of ≤ 100 nm diameter, at the epithelial surface and within lung-surface structures. Macrophages contained also large AuNP agglomerates (> 100 nm). At 0 h after aerosol inhalation, 69.2±4.9% AuNP were luminal, i.e. attached to the epithelial surface and 24.0±5.9% in macrophages in Scnn1b-Tg mice. In Wt mice, 35.3±32.2% AuNP were on the epithelium and 58.3±41.4% in macrophages. The percentage of luminal AuNP decreased from 0 h to 24 h in both groups. At 24 h, 15.5±4.8% AuNP were luminal, 21.4±14.2% within epithelial cells and 63.0±18.9% in macrophages in Scnn1b-Tg mice. In Wt mice, 9.5±5.0% AuNP were luminal, 2.2±1.6% within epithelial cells and 82.8±0.2% in macrophages. BAL-macrophage analysis revealed enhanced AuNP uptake in Wt animals at 0 h and in Scnn1b-Tg mice at 24 h, confirming less efficient macrophage uptake and delayed clearance of AuNP in Scnn1b-Tg mice.

Conclusions

Inhaled AuNP rapidly bound to the alveolar epithelium in both Wt and Scnn1b-Tg mice. Scnn1b-Tg mice showed less efficient AuNP uptake by surface macrophages and concomitant higher particle internalization by alveolar type I epithelial cells compared to Wt mice. This likely promotes AuNP depth translocation in Scnn1b-Tg mice, including enhanced epithelial targeting. These results suggest AuNP nanocarrier delivery as successful strategy for therapeutic targeting of alveolar epithelial cells and macrophages in COPD.

Keywords:
Aerosol; COPD; Electron microscopy; Gold nanoparticles; Inhalation; Lungs; Macrophages; Stereology